Vitaprofeno Forte
Efficacy test of Vitaprofeno Forte

Introduction

Inflammatory processes can be specific or non-specific like those which are commonly observed in sport horses after exertion. These non-specific post-exertion inflammatory processes basically involve the large muscular masses (myositis) and/or the ligaments and tendons of the legs (desmitis and tendinitis respectively).

There is an increase of pain sensation and blood chemistry shows an increase of plasmatic fibrinogen(the protein in the acute phase of inflammation) and the muscular enzymes, creatine kinase (CK), aminotransaminase asparatate (AST) and lactate dehydrogenase (LDH).

The increase in the plasmatic concentration of these enzymes can occur as a consequence of a muscular injury or as an increase in the permeability of the cell membrane of muscular cells because of the lowering of the pH. In the latter case, the increase is transient.

Objetive
To meassure the anti-inflammatory effect of Vitaprofeno in horses which undertook a test of maximum effort.

vita1

2

3

 

 

Basal

6 hr

12 hr

24 hr

48 hr

Control

225(250)

350(251,6)

500(408,2)

575(287,2)

450(369,7)

Treated

150(57,4)

350(368,7)

125(50)

200(141,4)

175(95,7)

 

Materials and Methods

Animals: Four horses were used, three mares and one stallion of 2 - 3 years, three Arabs and one Anglo-Arab. All the horses undertook the same training programme, were stabled and received the same food based on alfalfa hay (4kgs/ day) and oats (3kgs/ day).

Exercise Test
It begins with a pre-warming phase of 1min at 1.7m/s and at trotting speed 4min. at 4m/s without a slope.
Each phase of the test lasted 1 min, and the slope during the whole test was of 6%. It began with a speed of 5 m/s and it was increased until the animal showed fatigue (moment at which the horse can´t maintain the race speed). The last phase of the test is the recuperation stage, 4 min of trotting at 4m/s and 1 min at 1,7m/s without slope.

Equipment:
For the exertion test, an ergometric treadmill Kagra Mustang 2200 was used. The enzymograms (CK and LDH) and the measurement of the plasmatic fibrinogen were done with an automatic analyzer Aeroset of Abbot

Experiment design:
A Latin square was used. The horses were divided into 2 groups of 2 animals and they were tested as controls and as treated animals with 10 days difference between each test. Vitaprofeno was administered in the treated group 30 min. post-exertion test. The blood samples were obtained through vein puncture in the jugular vein at 8a.m. (basal sample) and at 6, 12, 24 and 48 hrs post-exercise test.

Results

  • The plasmatic CK concentration of both groups, increased up to it´s maximum peak at 6 hrs after ending the test. It then began to decrease and to reach basal concentrations between 24 and 48 hrs post-test.
  • After 48 hs the concentration of CK of both groups was lower than at the beginning of the test. It´s important to state, that the highest concentration of this enzyme never exceeded the maximum reference value (250 U/l) See Fig. 1
  • The LDH, the other enzyme used as an indicator of muscle injury, showed a similar behaviour to that observed with the CK.
  • The highest concentration in both groups was observed after 6 hrs, then it began to decrease, reaching the basal concentration after 48hrs. This enzyme has a high molecular weight, and so it can be considered as an indicator of muscular injury when the concentration maintains a higher level after the first 48hrs post-exercise. So, despite the increase 6 hs after the test, there is no muscular damage, as the values of the enzyme reach again the basal amount after 48 hs. See Fig. 2
  • The plasmatic fibrinogen, the protein which indicates acute inflammation, shows a different behaviour from 6hrs post-test onwards.
  • There is an increase post-6hrs in both groups which is higher than the reference values (up to 200g/dl). This is because in the treated group Vitaprofeno didn´t reach it´s highest effect yet.
  • From 6 hs. on, while the control group continued increasing the concentration of the enzyme, in the treated group, now under the effect of the drug, the concentration of fibrinogen was reduced to basal values (within the reference values) and maintained the same values throughout the sampling period.
  • On the other hand, table 1 shows how the control group, without the administration of Vitaprofeno, has a high standard deviation resulting from the big individual variation to the exercise test, while the treated group, due to the effects of the anti-inflammatory medicine, show greater uniformity in the results.
  • To conclude, although the test exercise did not injured muscular cells, the transitory increase in the concentration of the enzymes CK and LDH were due to the increase in the permeability of the plasmatic membrane of muscular cells as a consequence of the acidosis produced by the exercise. But it indeed produced an acute non specific inflammatory process mitigated by the application of Vitaprofeno.

 

Viterra
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