Efficacy test of CHROMACTIV
To evaluate if Chromactiv produces any kind of positive effects on the performance of sport horses.
The association of the different constituents of this product make of it an excellent muscular metabolic catalyst. Arginine and glucose have an anti-oxidant and lipotropic effect on the hepatic metabolism. Glycine acts against fatigue. Glucose helps the replacement of the energy deposits of muscular glycogen; arginine and carnitine stimulate ß-oxidation and together with glycine, produce an anabolic effect. Chromium increases the tolerance of glucose and reduces lactic acidosis, delaying the appearance of muscular fatigue. On the other hand it speeds up post-exercise recovery. Vitamins of B group and minerals of this product have an important function as coenzymes in energy and protein metabolisms of the organism, but with a special function on the musculo-skeletal and nervous systems where normal functioning is restored.
Materials y Methods
- Three healthy sport horses were used. They were evaluated before treatment, and then Chromactiv was administered. The dose was of 1ml/20 kg of body weight and was given daily 5 days before testing.
- The animals were evaluated after a week’s training and had 3 days of rest between both treatments.
- The animals were evluated by a standardized exercise test before and after receiving treatment.
- The test was carried out on a Treadmill and heart rate was measured by a cardio-tachometer. Oxygen consumption was recorded by an ergospirometer and lactate was measured by accusport. Hemogram and enzymogram analyses were performed by an Abbot Aerocet auto-analyzer.
The animals trained six times a week on an ergometric treadmill, doing exercise of diferent distance and intensity each day. The horses were evaluated before and after the 5 days of receiving CHROMACTIV.
The test consists of 1 min at 1.7 m/s; 4 min at 4 m/s and stages of 1 min with increasing speed of 1 m/s till fatigue. To recover : 4 min at 4 m/s and 1 min at 1.7 m/s. All with a slope of 6%.
The measured parameters
During the test, heart rate, oxygen consumption (VO2), ventilatory equivalent (Eq. O2) and respiratory volume per minute (VE) were recorded. The time of recovery was also taken into account. An enzymogram was carried out 24 hours post-exercise to show whether there was any muscular injury (Creanquitinase – CK – and fibrinogen).
The results of VO2 max, VE and Eq.O 2 are shown on separate sheets for a better analysis. The heart rate is not shown as there were not any appreciable differences.
The results show that the 3 horses did an additional stage of the test during the Chromactiv period. That increase is supported with:
- VO2 max: Chromactiv increases the same. The value of horse Nº3 was without change but this was in a higher stage of the test.
- VE: Horse No. 1 shows a lower VE throughout the test which together with a best Eq.O 2 shows a compensation in breathing. Horse No. 2 has a VE without changes between both periods, but a lower Eq O2 is observed during the Chromativ period and shows that it obtained a very significant breathing efficiency. Horse No. 3 shows a similar VE and Eq.O 2 when the stages of greater effort of each test are compared.
- Hematocrit: A slight increase is observed after Chromativ administration. This allows an improvement in blood oxygen transportation.
- Lactate during V200 (speed developed at 200 heart beats/minute): All horses show a slightly higher value during the period with Chromativ showing an increase in anaerobic metabolism.
The recovery post-effort was measured with following values:
- CK: Only horse No. 1 showed an important decrease, but we need to emphasize that having done a stage more of the test during Chromativ period and not showing an increase of CK demonstrates a decrease of muscle cell membrane´s permeability.
- Fibrinogen: An increase is observed which is related to the increase in physical effort during testing in the Chromativ period. However both groups show values at usual rates.
- Recovery time: An improvement in recovery time is observed during the Chromativ period which is made more obvious bearing in mind the greater physical effort during this stage.
To conclude, Chromativ shows an improvement in performance by producing an increase in breathing efficiency, a larger consumption of oxygen and a larger contribution of the anaerobic metabolism which together with the larger VO 2 max delays fatigue. At the same time a better post-effort recovery is observed.